| 학회 |
한국고분자학회 |
| 학술대회 |
2011년 가을 (10/06 ~ 10/07, 김대중 컨벤션센터) |
| 권호 |
36권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Fabrication of chitosan microspheres containing fluorescently-labeled Bovine serum albumin (BSA) by electrospinning |
| 초록 |
In the current study, chitosan microsphere was prepared by electrospun chitosan solution at high voltages, which was followed by an instant solidification in sodium carbonate solutions. The fabricated microsphere was characterized by optical microscopy, scanning electron microscopy (SEM) and Fluorescence spectrometer. Average diameters of chitosan microspheres were significantly decreased in proportion to applied voltage and viscosity of chitosan solution. Fluorescently-labeled bovine serum albumin was encapsulated in the chitosan microsphere by electrospinning a mixture of chitosan and BSA solutions. The electrospun microsphere showed high loading efficiency of BSA because of electrostatic force between cationic chitosan and anionic and immediate solidification of the chitosan microspheres. Therefore, electrospinning can be an alternative fabrication method of microspheres in aims to minimize protein denaturation as well as simplify preparation steps. |
| 저자 |
김영희, 최지숙, 김혜성, 손영주, 윤수진, 조은주, 유혁상
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| 소속 |
강원대 |
| 키워드 |
microsphere; anionic protein; oral delivery
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| E-Mail |
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