| 초록 |
Fabrication of bone substitutes, which are a combination of bio-ceramics and bio-polymer, is performed to meet the demand for bone regeneration after fracture or disease. In this study, Hyaluronic Acid (HA)- Sodium CarboxymethylCellulose (NaCMC) where the ratio of HA and NaCMC was 2:1 used as 5% wt polymer concentration and Biphasic Calcium Phosphate(BCP) loaded as 2% wt and then 5% wt EDC crosslinker was added into the hydrogel complex. An aqueous solution of citric acid 1 wt% was then added as catalyst to the polymer solution, in a 5% vol/vol amount. The crosslinking reaction occurred in few hours at room temperature. After that the hydrogel was freeze-dried for 24 hrs for obtaining porous structure. Detailed morphological and material characterization, such as porosity, micro-structural analysis, chemical constituents of hydrogel scaffold was carried out. PBS uptake and degradation behavior of the hydrogel were also observed. Cell viability, cell attachment, and proliferation of pre-osteoblast MC3T3-E1 cells on the hydrogel were evaluated by the MTT assay, live-dead assay, and immunofluorescent. |
