| 학회 |
한국공업화학회 |
| 학술대회 |
2019년 가을 (10/30 ~ 11/01, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
23권 2호 |
| 발표분야 |
포스터_생물공학 |
| 제목 |
Liver cancer diagnosis based on total serum protein fingerprinting using MALDI-TOF-MS |
| 초록 |
As clinical tools for diagnosing cancer, ELISA or LC-MS-based methods have been conventionally applied. However, these methods are inappropriate for high-throughput analytical platforms because of inferior multiplexing capabilities, high development costs and long turnaround times. Here, we developed MALDI-TOF MS-based method for cancer diagnosis. Serum samples were briefly diluted with distilled water and then spotted on MALDI plates. Each of the integrated main spectrum profiles (MSPs) was generated by the MALDI Biotyper 3 software. The reliability of the integrated MSPs was successfully evaluated in a blind test set. This highthroughput method may be an effective platform technology for various cancer diagnosis and disease evaluations. |
| 저자 |
김성민1, 송원석2, 조성현1, 이재승1, 전효진1, 김윤곤1
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| 소속 |
1숭실대, 2서울대 |
| 키워드 |
MALDI-TOF; liver cancer; fingerprinting; diagnosis
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| E-Mail |
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