| 학회 |
한국고분자학회 |
| 학술대회 |
2007년 가을 (10/11 ~ 10/12, 일산킨텍스) |
| 권호 |
32권 2호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
Surface Immobilization of Anti-Thrombin Aptamers on Si-Nanowire for Biosensor Applications |
| 초록 |
In this work, aptamer biosensor (aptasensor) gate was prepared to detect the optical and electronic signals during and after the chemical binding of biomolecules to aptamers on the chip. A silicon-nanowire (Si-NW) was used as a model substrate and anti-thrombin aptamer was used as a model aptamer. Surface modification was carried out using 3-aminopropyl diethoxysilane and succinic anhydride, or 3-trimethylsilylpropyl methacrylate to provide Si-NW with carboxyl or methacrylate groups. Aptamers with amine or thiol groups on 5’-end were chemically immobilized on the surface-modified Si-NW. Atomic force microscopic analysis confirmed the successful binding of anti-thrombin aptamers on Si-NW. The binding capacity of the aptamer biosensor to FITC labeled thrombin was successfully assessed with a fluorescent microscope. The thrombin aptasensor gate will be further applied for the real-time detection of electronic signals during and after thrombin binding. |
| 저자 |
김기수, 이현승, 조문호, 한세광
|
| 소속 |
포항공과대 |
| 키워드 |
Aptamers; Thrombin; Nanowire; Surface immobilization; Aptasensor
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| E-Mail |
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