| 학회 |
한국공업화학회 |
| 학술대회 |
2014년 가을 (11/12 ~ 11/14, 엑스코(대구)) |
| 권호 |
18권 2호 |
| 발표분야 |
(생물공학)Advances in nanobiotechnology |
| 제목 |
integrative Microarray Process for Antigen-specific Characterization of T lymphocytes (iMPACT) |
| 초록 |
CD4+ T helper cells play a central role in the immune response by adopting distinct functional patterns of cytokine release upon antigenic exposure. Exogenous factors such as cytokines modulate these patterns of functional response, suggesting that populations of cells can exhibit specific responses. However, since single-cell technologies have previously resolved only integrated measures of function, it remains unclear if the population-level response might arise in part from the dynamic modulation of responses by individual cells. Here, we used a single-cell co-culture assay to temporally resolve antigen-dependent cytokine responses exhibited by human CD4+ T cells ex vivo. The results demonstrate that the reported single cell assay can probe the profile of functional responses from individual antigen-reactive T cells persistently stimulated by antigen-presenting cells. |
| 저자 |
최종훈 |
| 소속 |
한양대 |
| 키워드 |
microarray; T cells; antigen; characterization; single cells
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| E-Mail |
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