| 초록 |
3D Bioprinting has attracted considerable attention for producing 3D engineered cellular microenvironments that replicate complex and sophisticated native ECM as well as the spatiotemporal gradients of numerous physicochemical and biological cues. Although various hydrogel-based bioinks have been reported, the development of advanced bioinks that can reproduce the complexity of ECM accurately and mimic the intrinsic property of laden cells is still a challenge. Herein, I present 3D printable bioinks composed of phenol-rich gelatin (GHPA) and graphene oxide (GO) which can form a hydrogel network in situ by a dual enzyme-mediated cross-linking reaction. The in situ curable GO/GHPA hydrogel can be utilized successfully as 3D-printable bioinks to provide suitable cellular microenvironments with facilitated myogenic differentiation of C2C12 skeletal myoblasts. Overall, it is suggested that functional bioinks may be useful in muscle tissue engineering and regeneration. |
