| 학회 |
한국화학공학회 |
| 학술대회 |
2005년 가을 (10/21 ~ 10/22, 인하대학교) |
| 권호 |
11권 2호, p.2098 |
| 발표분야 |
생물화공 |
| 제목 |
Transcriptome and fluxome analysis of a metabolically engineered E.coli L-threonine production strain |
| 초록 |
Rationally designed L-threonine production strain was derived from Escherichia coli to elucidate crucial constraints which is affecting the biosynthesis of L-threonine. The L-threonine production strain(LKH9) was constructed by releasing regulatory mechanisms such as feedback inhibition and attenuation. The LKH9 carries a deletion of lysA gene, metA gene, and tdh gene while a point mutation was introduced in ilvA gene to decrease the activity of a corresponding gene. Furthermore, the thrABC operon was amplified by using pKK223-3 plasmid. With the DO-regulated fed-batch fermentation, this rationally designed strain produces 22g/l of L-threonine. Transcriptome analysis was performed to investigate the global gene expression changes in a fed-batch cultivation. Metabolic flux analysis demonstrated that the carbon flux towards L-threonine biosynthesis was successfully increased. Our strategy not only successfully developed L-threonine production strain by rationally designed strain development, but revealed new constraints requiring high productivity. (This work was supported by Korean Systems Biology Research Grant, M10309020000-03B5002-00000 from the MOST.) |
| 저자 |
이광호1, 박진환2, 김태용2, 이상엽2
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| 소속 |
1한국과학기술원 생명화학공학과 / (주)CJ 바이오(연), 2한국과학기술원 생명화학공학과 |
| 키워드 |
L-threonine; metabolic engineering; transcriptome; fluxome
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| E-Mail |
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| 원문파일 |
초록 보기 |
