| 학회 |
한국고분자학회 |
| 학술대회 |
2009년 봄 (04/09 ~ 04/10, 대전컨벤션센터) |
| 권호 |
34권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
The evaluation of biocompatibility of Epoxy and PDMS in vitro as a implantable coating materials |
| 초록 |
Epoxy and polydimethylsiloxane (PDMS) are industrially important polymers used for the implantable coating material, according to their certified biocompatibility, sufficient mechanical strength and easy processibility. The cytotoxicity of these materials was evaluated using the MTT［tetrazolium dye, 2-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide, C18H16N5SBr］ assay. Materials extract was prepared following ISO 10993-5 procedure for preparing extracts of materials. We chose NIH-3T3 fibroblast cells because they represent well-characterized and established cell cultures for cytotoxicity assays. Positive controls were prepared by extracts from organo-tin based PVC, SDS solution and Triton-X 100 solution. Two different methods were adopted for the MTT test: 1) seeding cells with extracts 2) incubation extracts with cell sheets. In the presentation, both cell morphology and MTT numberal data will be shown for the comparison. |
| 저자 |
박수범, 이종환, 나경아, 정재연, 김명진, 현진호
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| 소속 |
서울대 |
| 키워드 |
Cytotoxicity test; Biocompatibility test; MTT; implantable coating polymer
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| E-Mail |
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