| 초록 |
Nanoparticles are useful for delivering therapeutics into cells. However, size, shape, surface chemistry and the presentation of targeting ligands on the surface of nanoparticles can affect circulation half-life and biodistribution, cell specific internalization, excretion, toxicity, and efficacy. A variety of materials have been explored for delivering small interfering RNAs (siRNAs). However, conventional delivery nanoparticles such as liposomes and polymeric systems are heterogeneous in size, composition and surface chemistry, and this can lead to suboptimal performance, lack of tissue specificity and potential toxicity. Here, we show that self-assembled DNA nanostructures with a well-defined size can deliver siRNAs into cells and silence target genes in dose dependent-manner. Various strategies to prepare the DNA nanostructures as well as the ligand-RNA conjugate system to facilitate the intracellular delivery will be presented. |
