| 학회 |
한국재료학회 |
| 학술대회 |
2016년 가을 (11/16 ~ 11/18, 경주 현대호텔) |
| 권호 |
22권 2호 |
| 발표분야 |
B. 나노화학/바이오 재료 분과 |
| 제목 |
The electrophysiological monitoring of aquaporin-4 and the real-time detection of its antibody using nanovesicle-based platform |
| 초록 |
Aquaporin-4 (AQP4) water channel protein transports water molecules across cell membranes bidirectionally and involves in a neurological disorder, neuromyelitis optica (NMO) caused by anti-AQP4 antibodies. Here, we developed a platform based on nanovesicle-carbon nanotube hybrid nanostructures for the real-time detection of anti-AQP4 antibodies and the electrophysiological monitoring of AQP4 activities. Using the hybrid device, we could detect anti-AQP4 antibodies with a high sensitivity and estimate the binding constants under different osmotic conditions. The results show AQP4 had a better affinity to anti-AQP4 antibodies under hyper-osmotic conditions than normal conditions. Furthermore, our device can be utilized to study the real-time cellular responses related with AQP4 such as those to different osmotic stresses. This nanovesicle-based platform can be a simple but versatile tool for basic research about AQP4 and related biomedical applications such as disease diagnostics. |
| 저자 |
Cho Dong-guk1, Eun Jin Park1, Juhun Park1, Hyun Seok Song2, Sung Joon Kim1, Kyeong Cheon Jung3, Sung-Min Kim3, Daesan Kim1, Kyung Seok Park4, Seunghun Hong1
|
| 소속 |
1Seoul National Univ., 2Korea Institute of Science and Technology, 3Seoul National Univ. Hospital, 4Seoul National Univ. Bundang Hospital |
| 키워드 |
<P>Nanovesicle-based biosensor; Aquaporin-4; Nanovesicle-carbon nanotube hybrid nanostructure; Real-time detection; Osmotic stress</P>
|
| E-Mail |
|
