| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 봄 (04/14 ~ 04/15, 전경련회관) |
| 권호 | 30권 1호, p.268 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | bFGF delivery system using heparinized thermosensitive hydrogel for cartilage repair |
| 초록 | The interaction of heparin with specific proteins (growth factors and cytokines) facilitates a wide range of fundamental biological processes including cellular proliferation, differentiation and tissue homeostasis. The basic fibroblast growth factors, FGF-2, is well characterized that it is a potent modulator of cell proliferation, motility, differentiation, and survival, as well as plays an important role in normal regeneration processes in vivo, i.e. embryonic development, angiogenesis, osteogenesis, chondrogenesis, and wound repair. bFGF specifically binds to heparin with a high affinity, and both its mitogenic activity and biological stability are modulated by heparin. Heparin also protects bFGF from inactivation by acid and heat, as well as from degradation by proteases. Therefore, heparin immobilized biomaterials have a lot of potential for tissue regeneration as bFGF controlled delivery vehicle and biocompatible material. In this study, Tetronic-PLA-heparin conjugate was prepared by coupling of heparin with Tetronic-PLA copolymer to improve their growth factor affinity and biocompatibility. Tetronic-PLA copolymer was synthesized by ring opening polymerization of L-lactide with Tetronic using stannous octoate. Then, heparin was coupled to Tetronic-PLA copolymer by 1-ethyl-3-(3-dimethylamino propyl)-carbodiimide (EDC) and N-hydroxysuccinimide (NHS) in MES buffer. Tetronic-PLA-heparin conjugate obviously demonstrated the presence of PEO, PPO, PLA and heparin blocks at FT-IR and 1H-NMR spectra. Tetronic-PLA-heparin was characterized by GPC, DLS and colorimetric assay and the thermo-responsive behaviors of aqueous polymer solution were investigated. (Fig. 1) Tetronic-PLA-heparin demonstrated a higher affinity with bFGF than other samples. Furthermore, Tetronic-PLA-heparin conjugate showed significant sustained release behaviors over 100hr, while the control showed a rapid bFGF release of more than 90% (Fig. 2.). In vitro cell study is under investigation. These results suggest that Tetronic-PLA-heparin conjugate would be very useful in bFGF delivery applications as an injectable material for tissue regeneration. Fig. 1. Phase transition diagram of (a) Tetronic, (b) Tetronic-PLA (c) Tetronic-PLA-heparin Fig. 2. bFGF release profile of (a) Tetronic-PLA-heparin (b) unheparinized Tetronic-PLA |
| 저자 | 고동현1, 이준우2, 박기동1 |
| 소속 | 1아주대, 2한국과학기술정보(연) |
| 키워드 | bFGF; thermosensitive; hydrogel; injectable |
