| 초록 |
Hypoxia is caused in tumors because oxygen supplied by blood is not enough for the tumor tissue. The treatment efficacy of radiotherapy and chemotherapy is known to be reduced by hypoxic environment of cancerous tissues. Hypoxia may also lead to metastasis of the tumor. One solution to reverse the hypoxic environment is by introducing shelled microbubbles which contains oxygen as a core gas. Microbubbles have been in use as ultrasound contrast agent for decades. Microbubbles can be used as oxygen carriers. Microbubbles can be injected intravenously. The high intensity ultrasound causes cavitation and which leads to the destruction of microbubbles and releases core gas. Diffusion across the concentration gradient may also help in dissolution of oxygen in hypoxic environment. The oxygen microbubbles synthesized in this work have lipid shells, giving them better biocompatibility. DSPC, DSPE-PEG and DSPE-Biotin were dissolved in chloroform and heated above lipid transition temperatures. The dried lipids were then dissolved in PBS solution and microbubbles were synthesized using sonication of the solution in which oxygen was continuously supplied. |
