| 학회 |
한국화학공학회 |
| 학술대회 |
2011년 봄 (04/27 ~ 04/29, 창원컨벤션센터) |
| 권호 |
17권 1호, p.350 |
| 발표분야 |
생물화공 |
| 제목 |
Trypsin Coated Magnetic Nanoparticles for Protein Digestion |
| 초록 |
Protein digestion is a critical step in bottom-up proteomic analysis, but takes a long time due to the poor stability of digestive enzyme called trypsin. In the present work, we prepared trypsin-coatings on magnetic nanoparticles (EC-TR/NPs) by performing a simple step of glutaraldehyde crosslinking. The resulting trypin-coated magnetic nanoparticles (EC-TR/NPs) were highly stable by showing negligible activity loss after recycled uses under continuous shaking for 27 days. Highly stable and magnetically separable EC-TR/NPs were successfully employed in protein digestion for proteomic analysis. |
| 저자 |
이병수1, 김중배1, Daniel Lopez-Ferrer2, Richard D. Smith2
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| 소속 |
1고려대, 2Pacific Northwest National Laboratory |
| 키워드 |
Nanobiocatalysis; Enzyme immobilization; Protein digestion; Pressure cycling technology
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| E-Mail |
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| 원문파일 |
초록 보기 |
