| 학회 |
한국화학공학회 |
| 학술대회 |
2011년 봄 (04/27 ~ 04/29, 창원컨벤션센터) |
| 권호 |
17권 1호, p.419 |
| 발표분야 |
생물화공 |
| 제목 |
Optimized Conditions for Recovery and Reuse of Immobilized Lysosomal Enzymes as an Antimicrobial Agents |
| 초록 |
As a tool for the stable enzyme reuse, enzyme immobilization skill has been investigated for several decades. Surface of magnetite nanoparticles coated with (3-aminopropyl) triethoxysilane (APTES) by a silanization reaction have been enhanced as a support for the immobilization of enzyme in this study. To immobilize the lysosomal enzymes , we examined through rotation method at room temperature for 2 hours optimized in this study, the immobilization efficiency, activity and recovery of lysosomal enzymes immobilized on magnetite to form amine group nanoparticles were evaluated. Among various volume ratio (w/w) of lysosomal enzymes and TiO2 nanoparticles, we found that 100 % of immobilization efficiency of was observed at a ratio of 1:5, 10 and 20 (enzymes:magnetite; w/w). Furthermore, the antimicrobial activities of the immobilized lysosomal enzymes were confirmed using viable cell counting methods against Escherichia coli. Immobilized lysosomal enzymes could be recovered by magnetic separation and showed the constant activity during recycles. |
| 저자 |
방승혁1, 김소정1, 김 필1, 김양훈2, 민지호1
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| 소속 |
1전북대, 2충북대 |
| 키워드 |
Lysosomal enzymes; Magnetite; Recovery
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| E-Mail |
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| 원문파일 |
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