| 초록 |
Biological production of cadaverine has been suggested as an alternative route to replace polyamides generated from the petroleum-based process. For an ideal bioprocess, cadaverine should be produced with high yield and productivity from various sugars abundant in biomass. However, most microorganisms are not able to efficiently metabolize other biomass-derived sugars as fast as glucose. This results in reduced growth rate and low carbon flux toward the production of desired bio-chemicals. Thus, we engineered Escherichia coli to produce cadaverine from galactose, a promising future feedstock. To achieve this, genes related to the metabolic pathway were maximally expressed via synthetic expression cassettes. Furthermore, the feedback inhibition and degradation/re-uptake pathways were inactivated. Finally, the resultant strain produced 8.80 g/L cadaverine with high yield (0.170 g/g) and productivity (0.293 g/L/h), which was similar to or better than the previous glucose fermentation. |
