| 학회 |
한국고분자학회 |
| 학술대회 |
2018년 가을 (10/10 ~ 10/12, 경주컨벤션센터) |
| 권호 |
43권 2호 |
| 발표분야 |
기능성 고분자 |
| 제목 |
Ni2+-NTA –polystyrene nanoparticle encapsulated Fe3O4 magnetic particles as an efficient tool for his6-protein purification |
| 초록 |
An efficient and simple strategy for the purification of recombinant proteins has been needed with increase of the various recombinant proteins for diverse applications. In this work, we developed Ni2+-NTA-surface functionalized polystyrene nanoparticle encapsulated with Fe3O4 NPs (Fe3O4@Ni2+-NTA-PS) via miniemulsion/solvent evaporation method for the affinity purification of his6-tag recombinant proteins. The Ni2+-NTA end allows immobilization of his6-proteins, whereas the PS backbone provides hydrophobic environment for the encapsulation of magnetic Fe3O4 NPs. The spherical morphology, nano-size and magnetic property of the particles were characterized by DLS, TEM, SEM and VSM. The protein binding and recovering efficiency were estimated using Bradford assay, PL spectroscopy and SDS-PAGE. The practical application was demonstrated by separating the his6-proteins from crude cell lysate by applying magnet. |
| 저자 |
리자 조스1, 이채연1, 황아란1, 박지현2, 송재광2, 백현종1
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| 소속 |
1부산대, 2한국화학(연) |
| 키워드 |
Ni2+-NTA; functionalized polystyrene; Fe3O4 encapsulation; miniemulsion; his6-tagged protein; cell lysate purification
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| E-Mail |
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