| 초록 |
Calcium phosphate such as hydroxyapatite (HA), tricalcium phosphate(TCP), and biphasic calcium phosphate(BCP) compose the majority of mineral content of bone tissue. These calcium containing compounds are known for their strength, biodegradation, and biocompatibility which makes them suitable as bone graft materials. TCP is capable of releasing large amount of calcium ions which makes it highly degradable and there for more ideal for augmenting mineralization of regenerating bone. On the other hand HA has a highly stable composition which makes it last longer than TCP and therefore is more ideal as bone tissue scaffold. BCP is a combination of TCP and HA thus it has both the strength and weakness of each and would behave accordingly depending on the ratio of its constituents. Although several studies have been conducted using these materials early, middle, and late stages osteoblastic differentiation processes on TCP and BCP are not yet understood accurately. In this study biodegradation, biocompatibility and bone tissue regeneration capacity of TCP and BCP were examined in vitro and in vivo respectively. These compounds were characterized through x-ray diffraction (XRD, Energy-dispersive X-ray spectroscopy (EDS), degradation analysis, and pH measurement. Analysis of western blot and RT-PCR of cell extracts from pre-osteoblasts seeded on BCP scaffolds show significant change in alkaline phosphatase (ALP), osteocalcin (OCN), osteopontin (OPN) gene expression compared to cells cultured on TCP scaffolds. Histological examination of rabbit tibia implants revealed bone formation and maturation were better in BCP scaffolds compared to their TCP counterpart. This study is the first to compare the effects of TCP and BCP on new bone formation. |
