| 초록 |
The human skin equivalent (HSE) models are used as an alternative animal test of permeation and toxicity screening of chemicals. Here, we demonstrate digital light processing (DLP) three dimensional (3D) printing for the mimicry of the HSE model. In comparison with traditional methods for constructing HSE, it offers advantages in terms of cell laden retention, reproducibility and high culture throughput. Primary human keratinocytes are incubated on a silk fibroin-PEG hydrogel scaffold containing fibroblasts and also grown at the air-liquid interface, which allows the enhanced maturation and stratification. In the presentation, the detailed process of purification of silk fibroin from the cocoon, the synthesis of acrylated-PEG, 3D printing of bioinks, and the critical parameters of the cell culture will be provided. |
