| 학회 |
한국고분자학회 |
| 학술대회 |
2008년 가을 (10/09 ~ 10/10, 일산킨텍스) |
| 권호 |
33권 2호 |
| 발표분야 |
기능성 고분자 |
| 제목 |
Smart Bead-based Platform of Immunoassay Using a Stimuli-responsive Biopolymer and a Removable Polymeric Template |
| 초록 |
We present a smart bead-based platform of immunoassay using an elastin-like polypeptide (ELP) conjugated microbeads and a removable polymeric template. To make a defined bead microarray, micropatterns of a removable polymeric template were transferred onto the spun-cast adhesive thin layer by microcontact printing. UV-curable adhesive was applied to enforce the adhesion of the microbeads at the substrate and its adhesion performance was controlled by UV irradiation time. The microarray of polystyrene bead conjugated with ELP was used for the smart immunoassay of prostate specific antigen (PSA), a cancer marker. By controlling the phase transition of ELP molecules, PSA molecules were selectively captured and separated from the mixture of biomolecules. This method can be successfully applied to a variety of substrates including metal, glass, polymers and ceramic to fabricate single or multiple microbead arrays. |
| 저자 |
이종환1, 정재연1, 나경아1, 김명진1, 박수범1, 허필우2, 현진호1
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| 소속 |
1서울대, 2한국기계(연) |
| 키워드 |
microbead; elastin-like polypeptide; immunoassay
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| E-Mail |
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