| 학회 |
한국고분자학회 |
| 학술대회 |
2021년 가을 (10/20 ~ 10/22, 경주컨벤션센터) |
| 권호 |
46권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 Ⅰ,Ⅱ |
| 제목 |
Hyaluronic acid decorated nanofiber fragment scaffold for corneal regeneration |
| 초록 |
Polymeric nanofibrous scaffold decorated by hyaluronic acid (HA) was prepared as scaffold providing cornea mimicking environment. For the fabrication of nanofiber fragment (NF), electrospun nanofiber was physically fragmented and chemically fragmented and functionalized by aminoylsis in 1M EDA / MeOH at 37 ℃ for 72 h. Size ＜ 160 m of NFs were collected by repeated sieving. NFs were incubated for 3h in 15% (w/v) methacrylated hyaluronic acid solution containing 0.1% (w/v) Irgacure 2959 and UV was irradiated to get HA decorated NF (HA@NFs). In the result of FTIR and TGA analysis of HA-PCL NF, increasing the UV irradiation time from 0 to 20 min positively affected swelling property and the HA contents on HA@NFs. When HA@NFs and human corneal epithelial cells (HCEC) were cultured together, they were self-assembled and promoted to cell-sheet. According to CLSM analysis, the cells in the HA@NF scaffold have shown higher cell adhesion and spread morphology. |
| 저자 |
조완호, 유혁상, Wei Mao, 박재근, Oanh-Vu Pham-Nguyen, 이미소
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| 소속 |
강원대 |
| 키워드 |
Hyaluronic acid; nanofiber fragment; 3D culture; corneal regeneration
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| E-Mail |
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