| 초록 |
Enzymatic synthesis of biodiesel from canola oil using different immobilized enzymes (Novozym 435, Lipozyme TL IM, Lipozyme RM IM) under supercritical carbon dioxide (ScCO2) has been studied. ScCO2 is especially favored among media, because it is non-toxic, nonflammable and suitable for easy separation of products (biodiesel and glycerol). The reaction conditions of pressure, enzyme loading amount (%), temperature, agitation speed, time and methanol/oil moral ratio were 100 bar, 35%, 50℃, 300 rpm, 3 hr, and 3, where methanol was added stepwise to alleviate substrate inhibition of the enzyme by methanol. Among tested enzymes, Lipozyme TL IM showed the highest conversion (86.9%) under the reaction conditions, showing potential as a low-cost biocatalyst for biodiesel production. |
