| 초록 |
The tumor suppressor protein p53 plays crucial role in disturbing the growth of cancer. The damage of p53 function, which results almost every from mutations within the DNA binding domain, is related with human cancers. The collective electrons of the nanometer-sized metallic structures oscillate with the incident photon frequency which is denoted as a localized surface plasmon resonance. An λmax shift will occur in each step and we expect that the delta maxs to be different according to the different types of p53. resultingly, between the gadd45 promoter and p53 proteins affinity difference. The case of wild type, the mutation type is higher than the binding affinity. Also depending on the mutation, affinity appears differently. This result successfully explain a non-labeling detection system for biomolecular interaction and it possesses a great potential as a sensitive on-chip. |
