| 학회 |
한국고분자학회 |
| 학술대회 |
2014년 봄 (04/10 ~ 04/11, 대전 컨벤션센터) |
| 권호 |
39권 1호 |
| 발표분야 |
의료용 고분자 부문위원회(초청-영어발표/포스터-국문발표 가능) |
| 제목 |
Osteogenesis Differentiation of Rabbit Bone Marrow Mesenchymal Stem Cells on Porous Silk Scaffolds Fabricated by Loading Hydroxyaptite at Simulated Body Fluid Solution for Bone Tissue Engineering |
| 초록 |
In this study hydroxyapatite (HAp) were loaded in simulated body fluid (SBF) solution onto silk fibroin scaffold. The physicochemical properties of the materials were characterized by FTIR and compressive strength while scaffold morphology was investigated by scanning electron microscopy (SEM) with energy-dispersive spectroscopy to validate the process used for synthesis. Finally, the response of bone marrow mesenchymal stem cells (rbBMSCs) in terms of cell proliferation and differentiation to the osteoblastic phenotype was evaluated using the MTT assay, alkaline phosphatase activity, RT-PCR, and histological staining. The biological results revealed that the HAp/silk scaffolds are suitablefor the proliferation and differentiation of BMSCs in vitro and in vivo, supporting osteogenesis. This research was supported by NRF-2012M3A9C6050204, Bio-industry Technology Development Program (KMFAFF, 112007-05-2-SB010) and the Brain Korea 21 PLUS Project, NRF. |
| 저자 |
박진영, 국현, 김수민, 김혜민, 오승창, 김은영, 송정은, 강길선
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| 소속 |
전북대 |
| 키워드 |
Bone Marrow Mesenchymal Stem Cell(BMSC); Osteogenesis Differentiation; Bone Regeneration; Silk Fibroin; Hydroxyaparite(HAp); Simulated Body Fluid(SBF)
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| E-Mail |
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