| 학회 |
한국화학공학회 |
| 학술대회 |
2014년 가을 (10/22 ~ 10/24, 대전 DCC) |
| 권호 |
20권 2호, p.1558 |
| 발표분야 |
생물화공 |
| 제목 |
Production of Polyhydroxyalkanoates (PHAs) Containing 2-Hydroxybutyrate (2HB) by Metabolic Engineered Escherichia coli |
| 초록 |
PHAs are biodegradable and biocompatible compounds. The recombinant E. coli expressing evolved Clostridium propionicum propionyl-CoA transferase (PctCp) and Pseudomonas sp. MBEL 6-19 PHA synthase 1 (PhaC1Ps6-19) was developed and cultured in a chemically defined medium containing 20 g/L of glucose with various concentrations of 2HB and 3HB. Also, heterologous metabolic pathway to supply 2-HB from glucose was constructed via the citramalate pathway. Recombinant E. coli expressing the phaC1437, pct540, cimA3.7, and leuBCD genes together with the L. lactis II 1403 panE gene successfully produced PHAs consisting of 2HB, 3HB, and a small fraction of lactate by varying the 3HB concentration on MR medium. [This work was supported by the Technology Development Program to Solve Climate Changes (systems metabolic engineering for biorefineries) from the Ministry of Education, Science and Technology (MEST) through the National Research Foundation of Korea (NRF-2012-C1AAA001-2012M1A2A2026556) and by the Intelligent Synthetic Biology Center (2011-0031963) through the Global Frontier Research Program of the MEST.] |
| 저자 |
김제웅1, 김민경1, 박시재2, 송봉근1, 이승환3, 이상엽1
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| 소속 |
1KAIST, 2명지대, 3한국화학(연) |
| 키워드 |
PHA; 2HB; PLA; metabolic engineering
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| E-Mail |
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| 원문파일 |
초록 보기 |
