| 학회 |
한국공업화학회 |
| 학술대회 |
2010년 봄 (05/13 ~ 05/14, BEXCO(부산)) |
| 권호 |
14권 1호 |
| 발표분야 |
생물공학 |
| 제목 |
Bioconversion of lignin monomers to styrene derivatives using phenolic acid decarboxylase from Bacillus amylolichefachiens in two phase reaction system |
| 초록 |
Phenolic acid decarboxylase (PDC) catalyzes non-oxydatively p-coumaric acid to4-hydroxy styrenethatis useful for industrial application. Virtual screened PDCs have been over-expressed in Escherichia coli BL21(DE3), and purified PDCs were preparedto compare their activities.Kinetic studiesrevealed thatPDC from Bacillus amylolichefaciens showed a 1.5-fold higher specific activity than that ofreported Lactobacillus plantarum for p-coumaric acid. Productivity of recombinant E.coli strain shows 1.44 mmol/min/g (dcw). To extract 4-hydroxy styrene continuously during the reaction at high substrate concentration, biphasic reaction systemwere applied with n-hexane as organic solvent. |
| 저자 |
정다혜, 이나훔, 서주현, 최권영, 정은옥, 김병기
|
| 소속 |
서울대 |
| 키워드 |
p-coumaric acid; Lignin monomer; phenolic acid decarboxylases; two phase reaction
|
| E-Mail |
|
