| 초록 |
Clostridium beijerinckii is a gram-positive, spore-forming, obligate anaerobic bacterium, which is able to produce solvents acetone, butanol and ethanol by using various sugars. A gene expression reporter system (pCBEst-MTL) for C. beijerinckii NCIMB 8052 was developed by using the esterase gene from Clostridium as a reporter gene. In order to test the reporter system, promoters of key metabolic pathway genes, ptb (coding for phosphotransbutyrylase), thl (coding for thiolase), glnA (coding for glutamine synthetase) from C. beijerinckii NCIMB 8052, were cloned upstream of the reporter gene in pCBEst-MTL to construct plasmids pCBEst-MBP, pCBEst-MBT, and pCBEst-MBG, respectively. Detection of esterase activity performed with strains NCIMB 8052 (pCBEst-MBP), NCIMB 8052 (pCBEst-MBT), and NCIMB 8052 (pCBEst-MBG) demonstrated that each reporter gene expression system produced functional esterase in C. beijerinckii. |
