| 초록 |
A new polypeptide-based reverse thermogelling biomaterial has been developed. The sol-to-gel transition was observed at less than 10 wt. % aqueous solution. The mechanism of the sol-to-gel transition was investigated using FTIR, circular dichroism, NMR, etc. The change in conformation of polypeptide as well as PEG was involved during the sol-to-gel transition. The polymer was degraded in in-vivo, whereas it was stable in PBS. The current polypeptide must be very promising for biomedical applications including tissue engineering, cell/stem cell therapy, and drug delivery due to the fact that 1) it has in-situ gel forming ability, 2) pH drop was not observed during the degradation of the polymer, 3) the polymer is stable in buffer, however, degraded after in-vivo application, and 4) a low polymer concentration is needed to form a robust gel. |
