| 초록 |
Oral vaccination has been considered as a convenient strategy compared with other conventional means. However, this method poses some problems, such as low antigen stability, and low targeting efficiency. To resolve those, we modified the MCM-48 nanoparticles as oral vaccine delivery carriers. For enhanced delivery to the M cells, polyethylene glycol (PEG) was grafted on the surface of the particles. Then ovalbumin (OVA) was impregnated as a model antigen (i.e., OVA/PEG-MCM-48). To protect the OVA while passing the gastric cavity, those particles were enteric coated (i.e., OVA/ED-PEG-MCM-48). The confocal image revealed that PEG-MCM-48 could be well localized in M cells. The in vitro OVA release study exhibited that the OVA/ED-PEG-MCM-48 contained most of the OVA without a significant release at simulated gastric condition, but could be sustained released at simulated intestinal condition. From these data, we propose the ED-PEG-MCM-48 as a potential carrier for oral vaccine delivery. |
