| 학회 |
한국공업화학회 |
| 학술대회 |
2007년 가을 (11/02 ~ 11/03, 한경대학교) |
| 권호 |
11권 2호 |
| 발표분야 |
고분자 |
| 제목 |
Synthesis and Applications of (Nitrilotriacetic Acid)-End-Functionalized Polystyrene |
| 초록 |
A new approach to bio-conjugate proteins and polymers will be discussed. The developed method is based on the strong interaction with a sequence of histidine residues (His-tag) in genetically engineered proteins and Ni2+ complexed nitrilotriacetic acid (NTA) group at the polymer chain end. His-tagged GFP was expressed in recombinant E. coli BL21 (DE3) and end-functionalized polystyrene was prepared by Atom Transfer Radical Polymerization using NTA containing initiator. As His-tagged proteins is well-known to have specific binding affinity with Ni2+-NTA, His-tagged GFP was able to be conjugated with Ni2+-NTA end-functionalized polystyrene in a controlled fashion. |
| 저자 |
김수정1, 이효경2, 이지연1, Hong Y. Cho2, 김유진1, Euidock Ryu2, 김상욱1, 이선구2, 백현종3
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| 소속 |
1Department of Polymer Science & Engineering, 2Pusan National Univ., 3Department of Chemical Engineering |
| 키워드 |
bio-conjugate proteins; ; ATRP; end-funtionalized polystyrene
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| E-Mail |
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