| 학회 | 한국화학공학회 |
| 학술대회 | 2006년 봄 (04/20 ~ 04/21, 대구 인터불고 호텔) |
| 권호 | 12권 1호, p.298 |
| 발표분야 | 분리기술 |
| 제목 | Removal of protein using the aqueous two-phase system in the microfluidic device |
| 초록 | Serum albumin is one of abundant serum proteins which block the expression other important biomarkers. The objective of this study is to remove serum albumin effectively by using aqueous two-phase system in microfluidic devices. Aqueous two-phase extraction is biocompatible and widely used for separation of biomolecules. Aqueous two-phase system was applied to the microfluidic device for protein separation because relative low interfacial tension between top and bottom phase makes extraction process possible even at low flow rate and it induced no physical changes of polymer-based microchannel including polydimethylsiloxane microchannels. In this study, aqueous two-phase system was carried out with the effect of width, baffle, flow rates and electric field for the better BSA extraction efficiency in the microfluidic device. This work was supported by Grant No.R01-2004-000-10681-0 from the Basic Research Program of the Korea Science & Engineering Foundation. |
| 저자 | 이은주1, 이완희1, 허윤석1, 홍연기2, 홍원희1, 김도현1 |
| 소속 | 1한국과학기술원 생명화학공학과, 2충주대 |
| 키워드 | aqueous two-phase; microfluidic device |
| 원문파일 | 초록 보기 |
