| 학회 | 한국고분자학회 |
| 학술대회 | 2003년 가을 (10/10 ~ 10/11, 부경대학교) |
| 권호 | 28권 2호, p.179 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Chondrogenic differentiation of human bone marrow-derived mesenchymal stem cells and rat muscle-derived stem cells in 3D structures. |
| 초록 | Damaged articular cartilage is well known to have a limited potential for repair or regeneration due to avascularity and the presence of relatively few cells with low mitotic activity. Bone marrow derived mesenchymal stem cells (BMSCs) and muscle derived stem cells (MDSCs) may represent a convenient source of stem cells for cell-mediate gene therapy and tissue engineering application. Also, they have both self-renewal capabilities and multipotentiality. In this study, we have demonstrated the chondrogenic potential of human BMSCs and rat MDSCs under 3D cell culture conditions, provided that growth factors and other beneficial ingredients are present. In vitro, BMSCs and MDSCs were seeded in polymeric scaffolds for 28 days. The chondrogenesis of BMSCs and MDSCs was characterized by RT-PCR, ALP, MTT assay and staining with hematoxylin-eosin, safranin-O. We have confirmed expression of cartilage specific marker genes such as type Ⅱ collagen and aggrecan by RT-PCR. Acknowledgment: This work was supported by MOWH (IMT-2000, 01-PJ11-PG9-01NT00-0011). ∥ |
| 저자 | 김은정1, 박기숙1, 장지욱2, 이상진3, 김문석3, 조선행3, 한창환4, 이해방3, 강길선1 |
| 소속 | 1전북대, 2부경대, 3한국화학(연), 4가톨릭의과대 |
| 키워드 | BMSCs; MDSCs; chondrogenic differentiation; 3D structures |
