| 학회 | 한국고분자학회 |
| 학술대회 | 2003년 가을 (10/10 ~ 10/11, 부경대학교) |
| 권호 | 28권 2호, p.70 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Effect of small intestine submucosa (SIS) on osteoinduction of muscle-derived stem cells using SIS/PLGA scaffolds. |
| 초록 | Abstract : Current tissue engineering techniques require the use of a porous biodegradable/bioresorbable scaffold, which serves as three-dimensional template for initial cell attachment and subsequent tissue formation, both in vitro and in vivo. Small intestinal submucosa (SIS) has been investigated as a source of collagenous tissue with the potential to be used as a biomaterial because of its inherent strength and biocompatibility. SIS-loaded PLGA scaffolds were prepared by solvent casting/particle leaching. Characterizations of SIS contained PLGA scaffold were analyed by SEM, mercury porosimeter, and so on. Muscle-derived stem cells (MDSCs) can be differentiated in culture into osteoblasts, chondrocytes, and even myoblasts. Cellular viability and proliferation were assayed by MTT test. Osteogenic differential cell were analyed by alkaline phosphate activity, and RT-PCR. SIS/PLGA scaffolds were implanted into the back of athymic nude mouse to observe the effect of SIS on the osteoinduction compared with control PLGA scaffolds. Thin sections were cut from paraffin embedded tissues and histological sections were stained H&E, von Kossa, and immunohistochemical staining for TypeⅠcollagen and osteocalcin. Acknowledgement : This work was supported by MOWH(IMT-2000, 01-PJ11-PG9-01NT00-0011). |
| 저자 | 장지욱1, 양대혁2, 박기숙2, 김은정2, 이상진3, 이봉1, 한창환4, 김문석3, 조선행3, 이해방3, 강길선2 |
| 소속 | 1부경대, 2전북대, 3한국화학(연), 4가톨릭의과대 |
| 키워드 | small intestine submucosa; scaffold; muscle-derived stem cells; osteoinduction |
