| 학회 | 한국화학공학회 |
| 학술대회 | 2010년 봄 (04/22 ~ 04/23, 대구 EXCO) |
| 권호 | 16권 1호, p.375 |
| 발표분야 | 생물화공 |
| 제목 | Use of enzyme microreactor for the synthesis of alpha-ketoglutarate |
| 초록 | α-Ketoglutarate (also called oxo-glutarate) is an important nitrogen acceptor in metabolism. It is produced by oxidative deamination of glutamate or reductive carboxylation of succinate. It has been used for the production of creatin-α-ketoglutarate which used as a fitness supplement. In this study, we developed an efficient α-ketoglutarate production system using enzyme aggregated nanofibers and microreactor. L-glutamic dehydrogenase was aggregated on the surface of nanofiber and used to develop microreactor. The micro channel in a microreactor was covered with enzyme aggregated nanofibers and the reaction temperature was controlled at 45oC. When the flow rate was controlled at 0.3 ml/hr, the conversion of α-ketoglutarate from glutamate was 35%. |
| 저자 | 안영덕, 이중헌 |
| 소속 | 조선대 |
| 키워드 | L-glutamic acid; α-ketoglutarate; L-glutamic dehydrogenase; Immobilization; microreactor |
| 원문파일 | 초록 보기 |
