| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 봄 (04/14 ~ 04/15, 전경련회관) |
| 권호 | 30권 1호, p.669 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Preparation and Cell Adhesion Behaviors of Hydroxyapatite-Induced PLLA Films and Scaffolds for Tissue Engineering |
| 초록 | Bone fracture and damage are one of serious health problems. The current bone repairing or replacing materials include autografts, allografts, and various kinds of man-made materials such as polymers, bioceramics, metallic materials, and their composites. The poly(α-hydroxyesters) such as poly(glycolic acid) (PGA), poly(lactic acid-co-glycolic acid) (PLGA), and poly(L-lactic acid) (PLLA) satisfy many of these material requirements and have been fabricated into scaffolds for cell transplantation and tissue engineering. In this study, biodegradable PLLA nonporous films and dual pore scaffolds were prepared by using a solvent casting technique with chloroform and both a gas foaming and phase-separation methods, respectively. The obtained biodegradable film and dual pore PLLA scaffolds with hydrophobic surfaces were activated by using in situ direct plasma treatment for hydrophilic surface. The carboxylic acids onto PLLA surfaces were formed by acrylic acid (AA) graft plasma polymerization and then soaked in stimulated body fluid (SBF) from 1 to 20 days to induce hydroxyapatite (HA).1,2 The surface and bulk properties of PLLA films and porous scaffolds were measured by ATR-FTIR, ESCA, SEM, contact angle, XRD, and ICP. In addition, the adhesion and proliferation of fibroblast, chondrocyte, and osteoblast on some HA-formed PLLA films and scaffolds were evaluated. The surface of the films was more hydrophilic after AA grafting and hydroxyapatite formation whereas the surface morphologies of PLLA and PLLA-g-PAA films were similar but that of PLLA-g-PAA-HA film was significant different due to HA formation (Fig. 1). The order of cell adhesion on the PLLA films and scaffolds was PLLA control ＜ PLLA-g-PAA ＜ PLLA-g-PAA-HA. These biodegradable porous PLLA scaffolds are useful for intelligent matrix to regenerate artificial tissues and organs. PLLA control PLLA-g-PAA PLLA-g-PAA-HA (3 days) PLLA-g-PAA-HA (20 days) Fig 1. SEM morphology of some surface-modified PLLA films after immersion in SBF. References 1. H. Li and J. Chang, J. Mater. Sci., 15, 1089 (2004). 2. R. Zhang and P. X. Ma, J. Biomed. Mater. Res., 45, 285 (1999). |
| 저자 | 정현정1, 안광덕1, 안동준2, 한동근1 |
| 소속 | 1한국과학기술(연), 2고려대 |
| 키워드 | Tissue engineering; PLLA scaffold; Hydroxyapatite; Cell adhesion |
