| 학회 |
한국화학공학회 |
| 학술대회 |
2008년 가을 (10/23 ~ 10/24, 부산 BEXCO) |
| 권호 |
14권 2호, p.2760 |
| 발표분야 |
생물화공 |
| 제목 |
Production of fumaric acid by Rhizopus oryzae |
| 초록 |
Fumaric acid is afour-carbon unsaturated dicarboxylic acid andalso known as (E)-2-butenedioic acid or trans-1,2-ethylenedicarboxylic acid. The mycelia fungiRhizopus oryzae has been shown to be one of the most productive microorganisms in regard to the production of fumaric acid. It has extensive application in various industries, such as in the manufacture of sizing resins for the paper industry, as an acidulant in food industry, as a supplement in feed industry and as a promising candidate in the manufacture of polymer. The purpose of this study is to develop the medium for theselection of R. oryzae mutants and choosestrains for the higher production of fumaric acid. R. oryzae ATCC 20344 was grown in the flask containing YMS agar (4.1% YM agar, 0.5% NaCl and 0.2% MgSO4).Recoverd spore was treated with UV and successive γ-ray according to the lethal rate of 99.9%.Mutants were selectedbased on the halo size in the selection medium [2.1% YM broth (Difco), 0.1% Triton X-100, 0.06% Bromo cresolpurple (BCP) and 1.7% agar]. The production of fumaric acid inthe sumerged culture by R. oryzae mutantswas investigated. |
| 저자 |
강성우1, 이종호1, 김성봉1, 김승욱1, 김상용2, 이도훈2, 전계택3, 이명구4, 이하원4, 박철환4
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| 소속 |
1고려대, 2한국생산기술(연), 3강원대, 4광운대 |
| 키워드 |
fumaric acid; Rhizopus oryzae
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| E-Mail |
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| 원문파일 |
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