| 초록 |
Small interfering RNA (siRNA) has selectively recognize and cleave the target mRNA. Due to their innate physiochemical characteristics, however, the systemic delivery of naked siRNA fails to achieve therapeutically relevant gene silencing. To this end, a variety of cationic polymers and lipids have extensively studied for efficient siRNA delivery. In this study, we introduced nucleic acid aptamers on siRNA, which can enhance the cellular uptake. Aptamers sequence are directly synthesized with siRNA without coupling chemistry. In addition, DNA nanostructures were introduced for siRNA-aptamer (siRNA-Apt). DNA nanostructures can regulated density and location of siRNA-ligands in small area and can alter the Pharmacokinetics in vivo. siRNA-Apt showed enhanced cellular uptake efficiency. Also, when siRNA-Apt conjugates was hybridized with DNA nanostructure (holiday, tetrahedron), cellular uptake and target gene knockdown efficiency increased than only siRNA-Apt. |
