| 학회 |
한국화학공학회 |
| 학술대회 |
2020년 가을 (10/14 ~ 10/16, e-컨퍼런스) |
| 권호 |
26권 1호, p.333 |
| 발표분야 |
생물화공 |
| 제목 |
Tuning the Coenzyme B12 Riboswitch sensor with a transcriptional repressor to invert and amplify the signal |
| 초록 |
Genetic circuits are composed of input, logic, and output parts. Construction of complex circuits for practical applications requires numerous tunable genetic parts. However, the limited diversity and complicated tuning methods used for the input parts hinders the scalability of genetic circuits. Therefore, a new type of input part is required that responds to diverse signals and enables easy tuning. Here, we developed RNA-protein hybrid input parts that combine a riboswitch and orthogonal transcriptional repressors. The hybrid inputs successfully regulated the transcription of an output in response to the input signal detected by the riboswitch and resulted in signal inversion because of the expression of transcriptional repressors. Furthermore, the hybrid input detected both exogenous and endogenous signals, indicating potential applications in metabolite sensing. This hybrid input part could be highly extensible considering the rich variety of components. |
| 저자 |
황윤희1, 장성연2, 장성호3, 노명현1, 임현규4, 정규열1
|
| 소속 |
1포항공과대, 2포항공학대, 3인천대, 4캘리포니아대 |
| 키워드 |
생물공정 |
| E-Mail |
|
| 원문파일 |
초록 보기 |
