| 학회 |
한국공업화학회 |
| 학술대회 |
2020년 가을 (10/28 ~ 10/30, 광주 김대중컨벤션센터(Kimdaejung Convention Center)) |
| 권호 |
24권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Optimization of Metabolic Flux Rebalancing for Efficient Production of 3-Hydroxypropionic acid in Escherichia coli |
| 초록 |
3-Hydroxypropionic acid (3-HP) can be produced from glycerol by dehydration of glycerol to 3-hydroxypropionaldehyde (3-HPA) and oxidation of 3-HPA. The pathway imbalance between the rates of the two enzymatic reactions results in the accumulation of the toxic 3-HPA, which severely reduces 3-HP production. In this study, we used UTR engineering to increase the activities of glycerol dehydratase (GDHt) and aldehyde dehydrogenase (ALDH) for the high conversion of glycerol to 3-HP. Thereafter, the activity of GDHt was precisely controlled by varying expression of dhaB1, a gene encoding a main subunit of GDHt. The optimally balanced E. coli HGL_DBK4 showed enhanced 3-HP titer (40.51 g/L) and productivity (1.35 g/L/h). Especially, the yield of 0.97 g/g in a fed-batch experiment was the highest ever reported. Our study demonstrates that metabolic balancing through UTR engineering is an effective strategy for the development of efficient microorganisms for bio-refinery production. |
| 저자 |
강채원1, 정규열1, 임현규1, 노명현1, 박성훈2
|
| 소속 |
1포항공과대, 2부산대 |
| 키워드 |
3-hydroxypropionic acid; synthetic biology; metabolic engineering; balancing enzyme activity
|
| E-Mail |
|
