| 학회 | 한국공업화학회 |
| 학술대회 | 2020년 가을 (10/28 ~ 10/30, 광주 김대중컨벤션센터(Kimdaejung Convention Center)) |
| 권호 | 24권 1호 |
| 발표분야 | 포스터-생물공학 |
| 제목 | Enhanced 3-hydroxypropionic acid production with engineered aldehyde dehydrogenase using a directed evolution strategy |
| 초록 | Biological production of 3-HP from glycerol by glycerol dehydratase (GDHt) and aldehyde dehydrogenase (ALDH) has been discovered to be efficient, showing higher yield and productivity than other metabolic pathways. Still, as the low activity of GDHt and ALDH remained as a major problem of conversion process, it is crucial to engineer these two enzymes for further enhancement. In this study, we performed a directed evolution approach on an ALDH library, particularly aldehyde-binding site library of alpha-ketoglutaric semialdehyde dehydro-genase (KGSADH), using a synthetic selection device based on 3-HP responsive transcription factor to optimize 3-HP production pathway. Only two serial cultures resulted in enrichment of strains showing increased 3-HP production, and an isolated KGSADH variant enzyme exhibited a 2.79-fold higher catalytic efficiency toward its aldehyde substrate than the wild type. |
| 저자 | 김동환1, 석주연1, 양진아2, 최상진3, 임현규1, 최은종3, 박성훈4, 유태현3, 정규열1 |
| 소속 | 1포항공과대, 2서울대, 3아주대, 4UNIST |
| 키워드 | 3-hydroxypropionic acid; Directed evolution; Transcription factor; Screening; Synthetic biology; Metabolic engineering |
