| 초록 |
Ralstonia eutropha H16 that found in both soil and water is a Gram-negative lithoautotrophic bacterium that can make use of CO2 and H2 as its sources of carbon and energy in the absence of organic substrates. We constructed the lithoautotrophic genome-scale model of R. eutropha H16 based on the annotated genome sequence with biochemical and physiological information. The stoichiometic model, RehMBEL1391, is comprised of 1391 reactions including 229 transport reactions and 1171 metabolites. Constraints-based flux analyses were performed to refine and validate the genome-scale metabolic model under environmental and genetic perturbations. [This work was supported by the Advanced Biomass R&D Center(ABC) of Global Frontier Project funded by the Ministry of Education, Science and Technology. Further supports by the World Class University Program(R32-2008-000-10142-0) of the MEST were appreciated.] |
