| 학회 | 한국화학공학회 |
| 학술대회 | 2008년 가을 (10/23 ~ 10/24, 부산 BEXCO) |
| 권호 | 14권 2호, p.2612 |
| 발표분야 | 분리기술 |
| 제목 | Separation characteristics of adenine and cytosinein high performance liquid chromatography (HPLC) |
| 초록 | The growing interest in recombinant DNA technology require the application of fast and reliable methods of utmost sensitivity and selectivity for the analysis of mucleic acid compounds. High performance liquid chromatography (HPLC) is effective analytical technique for the separation of complex mixture. Adenine and cytosine, that is the structure component of nucleic compounds were separated on C18 column in HPLC systems and detection was performed by UV absorption at 270 nm. To find adsorption isotherms of adenine and cytosine, we carried out pulsed input method (PIM) in C18 column. Adsorption isotherm is calculated by Aspen chromatography, and compared with the simulation for separation of adenine and cytosine. |
| 저자 | 이선희, 우두경, 이진희, 김인호 |
| 소속 | 충남대 |
| 키워드 | Adenine; cytosine; C18; HPLC |
| 원문파일 | 초록 보기 |
