| 초록 |
Wound healing is composed of matrix remodeling, re-epithelization, vascularization, and contraction phases. At present, there is no ideal substitute for wound healing in the market because of immune rejection and infection problem. The aim of this study is to modify an artificial dermal equivalent (DE) by adding blood vessels which differentiated from a three-dimensional cell mass (3DCM). For formation of a 3DCM, hASCs were cultured on a novel FGF2-coated surface for 2 days in normal growth media and 3DCMs were then seeded on DE made of fibroblast cells. 3DCM/DE complex was transplanted to the wounded dorsum of nude mouse and analyzed by examination with the naked eyes and immunohistochemical staining. The 3DCM/DE group was more vascularized, thicker re-epithelialization, smaller scar formation and less wound contraction compared with other groups. These results indicate 3DCM promote denser vascularization and anticipate wound healing much faster with a proper matrix organization. |
