| 초록 |
RNA interference(RNAi) technology can be applied for the treatment of malignant tumor cells, because it can target specific cells and induce apoptosis while minimizing direct effects on tumor specific protein expressing mRNA level.(e.g. siRNA) However, delivering siRNA to target cells problem due to the large reactivity of the RNA is still present. It introduces a particleization method with using a polymer with siRNA to solve the siRNA stability problems in SERUM condition, but there is no standardized considering cytotoxicity or interfering with the action of siRNA. In this study, we introduced a method of using calcium ion as a surface coater for RNA complex. Calcium is already abundant in the body for osteogenesis, neurochemical response et al. so there is no need to consider toxicity in vivo. Coating the RNA complex to determine the likelihood of a calcium ion, and then we analyzed for morphological changes of the complexes and to perform in vitro cell experiment on Hela cells. |
