| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 봄 (05/10 ~ 05/12, 광주 김대중컨벤센센터(Kimdaejung Convention Center)) |
| 권호 |
21권 1호 |
| 발표분야 |
생물공학 |
| 제목 |
Standardizing the assay procedures of cell-free synthesized cytosol-penetrating antibodies |
| 초록 |
Antibodies that penetrate and accumulate in cytosol can greatly enrich the options for treating various diseases. Assessment of the cell-penetrating activity of individual antibodies, however, is a time-consuming and complicated process involving the expression and functional assay of purified recombinant antibodies. In this study, we made an attempt to streamline theprocedures for studying cell-penetrating antibodies by integrating the techniques of cell-free protein synthesis and cell-based functional assay. In this new scheme, PCR-amplified genes of cell-penetrating antibodies are rapidly expressed in the reaction mixture for cell-free synthesis, which are then directly transferred to the culture of reporter cells that generate signal in response to the cell-penetrating antibodies. It was required a number of issues to be solved before this scheme was made to work, which will be discussed in this presentation. |
| 저자 |
Christy Catherine1, 김지선2, Devi Kasi1, 박유진1, 이경호1, 최동기2, 김용성2, 양현정1, 김동명1
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| 소속 |
1충남대, 2아주대 |
| 키워드 |
cell-free protein synthesis; cell-penetrating antibody
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| E-Mail |
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