| 학회 | 한국화학공학회 |
| 학술대회 | 2017년 가을 (10/25 ~ 10/27, 대전컨벤션센터) |
| 권호 | 23권 2호, p.1731 |
| 발표분야 | 생물화공 |
| 제목 | Preparation of thin film coated cell platform |
| 초록 | In the field of cell biology, Layer-by-Layer (LBL) assembly technique has been widely studied because of its simple preparation and multifunctional properties. Substrates such as polysaccharide, silicon, and graphene based LBL platform applications are widely studied however, live cell based LBL platform is not yet fully understood. Therefore, live cell based LBL platform is required to be investigated more. Herein we demonstrated live cell based LBL platform. Moreover, we confirmed that the charge surplus can be used to deposit alternating protein on outer surface of thin film. We have developed live cell-LBL platform by stacking layers of hyaluronic acid (HA) and Ploy-L-lysine (PLL). The results showed that 3 bilayers of HA/PLL provides 4±1 nm of thickness on cell surface. Live cell LBL platform showed complete degradability. Additionally, Adherent cells (AML 12) and non-adherent cells (PBMCs) were evaluated and visualized as cell core. Also we have investigated zeta potential, cell viability, SEM images, and confocal images after assembly of 3 bilayers of HA/PLL. Thus this live cell-LBL platform is useful for various applications in cell biology. |
| 저자 | 황장선, 최종훈 |
| 소속 | 중앙대 |
| 키워드 | 생물화공 |
| 원문파일 | 초록 보기 |
