| 초록 |
In recent years, stimuli-responsive targeting anticancer drug delivery system has been interesting field of study due to its minimum side effects. In this work, to synthesize dual-targeted stimuli-responsive drug carrier, we have designed Outer Membrane Protein A (OmpA) conjugated poly(N-isopropylacrylamide) (PNIPAm) microgels containing superparamagnetic magnetite nanoparticles (MNP). MNP incorporated PNIPAm were copolymerized with carboxylic acid-functionalized monomers, which can be reacted with amine group of the OmpA via N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC)/N-hydroxysuccinimide (NHS) reaction. OmpA targeting ligand has ability to specifically target certain brain cells, where conjugation with MNP incorporated PNIPAm hydrogel surface, preparation of dual-targeted hybrid hydrogel can be realized. To ensure the influence of target specific ligands, cellular uptake of the OmpA conjugated microgels in brain cells was performed. |
