| 초록 |
Genetically encoded fluorescent probes such as green fluorescent protein (GFP) from jellyfish Aequorea victoria, its engineered variants, and GFP-related proteins derived from other marine organisms have been widely used for a variety of biotechnological applications including in vivo imaging of cells and tissues and in vitro fluorescence labeling. A major drawback of the GFP is strict requirement of molecular oxygen as a cofactor for the synthesis of the respective chromophores. Compared to GFP-related proteins, flavin mononucleotide (FMN)–based fluorescent proteins (FbFPs) allow in vivo labeling and detection under anaerobic condition but the FMN–based fluorescent proteins have very low fluorescence intensity. To expand the utility of the FMN-based fluorescent proteins, we have constructed a complex library and successfully isolated FMN–based fluorescent proteins exhibiting significantly improved fluorescence intensity via flow cytometry-based screening. |
