| 학회 |
한국고분자학회 |
| 학술대회 |
2011년 가을 (10/06 ~ 10/07, 김대중 컨벤션센터) |
| 권호 |
36권 2호 |
| 발표분야 |
고분자 구조 및 분석－Scattering & Spectroscopy |
| 제목 |
Structures of Ferritin and Apoferritin in Solution : pH Dependent Disassembly and Reassembly |
| 초록 |
The pH-dependent structures of the ferritin shell (apoferritin, 24-mer) and the ferrihydrite core, under physiological conditions that permit enzymatic activity, were investigated by small-angle X-ray scattering (SAXS). The intact hollow spherical apoferritin and ferrihydrite core was stable over a wide pH range, 3.40-10.0 and 2.10-10.0, respectively. As the pH decreased from 3.40 to 0.80, apoferritin underwent stepwise disassembly by first forming a hollow sphere with two holes, then a headset-shaped structure, and, finally, rodlike oligomers. As the pH was increased from pH 1.96, the disassembled rodlike oligomers recovered only to the headset-shaped structure, and the disassembled headset-shaped intermediates recovered only to the hollow spherical structure with two hole defects. The pH-induced apoferritin disassembly and reassembly processes were not fully reversible, although they were pseudoreversible over a limited pH range, between 10.0 and 2.66 |
| 저자 |
김미희, 노예철, 안병철, 정성민, 진경식, 김경태, 정정운, 김진철, 권원상, 고용기, 이문호, 김영용, 권경호
|
| 소속 |
포항공과대 |
| 키워드 |
apoferritin; ferritin core; disassembly; reassembly; structural model; small-angle X-ray scattering
|
| E-Mail |
|
