| 초록 |
Nowadays, a number of therapeutic agents for osteoporosis are available and focused on inactivation of osteoclasts. However, unfortunately the long-term use of anti-osteoporotic medications is associated with serious side effects. In this study, we examined osteogenic activities of blue mussel-derived antioxidant peptides PIIVYWK and FSVVPSPK in mouse mesenchymal stem cells (MSCs). Two antioxidant peptides increased osteogenic biomarkers such as alkaline phosphatase, type I collagen, osteocalcin and mineralization. Treatment antioxidant peptides in MSCs resulted in the increased expression of BMP-2, p-Smad1/5, Runx2, Dlx5, and osterix as well as phosphorylation of MAPKs. Further investigation using noggin, BMP antagonist, and MAPK inhibitors, we found that antioxidant peptides PIIVYWK and FSVVPSPK modulated osteoblast differentiation through ERK1/2 and JNK1/2-dependent BMP signaling pathway in MSCs. |
