| 초록 |
Klebsiella pneumoniae naturally produces large amounts of 1,3-propanediol (1,3-PD) and 2,3-butanediol (2,3-BD) using glycerol as a carbon source. The ldhA and mdh genes in K. pneumoniae were deleted based on its in silico gene knockout simulation. The K. pneumoniae ΔldhA Δmdh strain produced 125 g/L of diols (2.0 g/L/h) in the lab-scale fermentation using high quality guaranteed reagent grade glycerol. To evaluate the industrial capacity of the mutant strain, a pilot-scale fermentation (5,000-L) was carried out using crude glycerol obtained from the industrial biodiesel plant. The pilot-scale fed-batch fermentation of the mutant strain produced 114 g/L of diols (70 g/L of 1,3-PD and 44 g/L of 2,3-BD), with a yield of 0.60 g diols/g glycerol, and a productivity of 2.2 g/L/h of diols. [This work was supported by the Industrial Strategic Technology Development Program (No. 10050407) funded by the Ministry of Trade, Industry & Energy (MOTIE, Korea).] |
